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Cellartis DEF-CS 500 iPS無外源3D懸浮培養(yǎng)基

傳統(tǒng)的2D培養(yǎng)系統(tǒng)無法支持放大培養(yǎng)（Scale-up）所需細(xì)胞數(shù)量級(jí)別（> 10⁹個(gè)細(xì)胞/處理）以維持細(xì)胞療法的增長(zhǎng)需求。3D懸浮培養(yǎng)是大規(guī)模生產(chǎn)多能細(xì)胞的可行經(jīng)濟(jì)有效的解決方案。盡管干細(xì)胞傳統(tǒng)上難以適應(yīng)懸浮培養(yǎng)，但CellArtis研究人員已經(jīng)開發(fā)出一種培養(yǎng)體系，可以在成分確定、無飼養(yǎng)層和無異源物條件下實(shí)現(xiàn)穩(wěn)定高效的生長(zhǎng)并維持多能性。Cellartis DEF-CS 500 iPS無外源3D懸浮培養(yǎng)基與生物反應(yīng)器系統(tǒng)配套，為創(chuàng)建大量高質(zhì)量、未分化的起始材料提供了理想的方法。該培養(yǎng)基的配方基于2D培養(yǎng)基Cellartis DEF-CS 500 Xeno-Free Culture Medium，2D培養(yǎng)的細(xì)胞可以輕松過渡到3D以實(shí)現(xiàn)可靠的放大。

組份

• Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics (Cat. No. Y30047)
• 500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o Antibiotics (Cat. No. Y30046; not sold separately)
• Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Additives (Cat. No. Y30048)
  • 500 µl DEF-CS Xeno-Free 3D Spheroid Additive 1 (1,000X)
  • 200 µl DEF-CS Xeno-Free 3D Spheroid Additive 2 (4,000X)
  • 400 µl DEF-CS Xeno-Free 3D Spheroid Additive 3 (500X)

自行購買試劑

• PBS Dulbecco's w/o Ca²⁺ & Mg²⁺ (D-PBS --/--)
• Accumax (Merck Millipore, Cat. No. SCR006)

可選抗生素和消化試劑

• Gentamicin (Thermo Fisher, Cat. No. 15750)
• Versene Solution (Thermo Fisher, Cat. No. 15040) (for transfer from monolayer culture)

細(xì)胞培養(yǎng)參數(shù)