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StemMACS ChondroDiff Medium is an optimized differentiation medium for the generation of chondrocytes from human mesenchymal stromal cells (MSCs). It is suitable for the analysis or quality control of the differentiation capacity of expanded MSCs, or in vitro studies into the processes involved in MSC differentiation to chondrocytes, including gene expression or protein profiling.

Chondrocytes play a vital role in the development and healing of bone as well as other structural tissues in the body. Therefore, chondrocytes are focus of tissue engineering research into diseases such as osteochondrodysplasias, non-union fractures, and osteoarthritis. The elucidation of the mechanisms of chondrocyte development and regulation (chondrogenesis) remains a crucial focus in such research areas, and the use of optimized culture media for the efficient and reproducible generation of chondrocytes is requisite.

Components
100 mL StemMACS ChondroDiff Media, human:

• with L-glutamine
• with fetal bovine serum (FBS)
• with phenol red
• without antibiotics

Specifications

• pH: 7.3–7.7
• Functionality assay: Chondrogenic differentiation of mesenchymal stromal cells (MSCs) determined by aggrecan staining.
• Sterility: Tested for sterility.
• Storage: Store protected from light at –20 °C. The expiration date is indicated on the label. If thawed upon arrival, immediately mix thoroughly, aliquot, and store at –20 °C until use.

Applications

• Analysis or quality control of differentiation capacity of expanded MSCs.
• In vitro studies of MSC chondrogenesis (e.g., gene/protein profiling).