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| 產(chǎn)地 | 日本 |
| 保存條件 | |
| 品牌 | 寶日醫(yī)TAKARA |
| 貨號 | WK593S |
| 用途 | 細(xì)胞培養(yǎng) |
| 組織來源 | |
| 細(xì)胞形態(tài) | |
| 是否是腫瘤細(xì)胞 | 否 |
| 保質(zhì)期 | 見說明書 |
| 器官來源 | |
| 免疫類型 | |
| 品系 | |
| 生長狀態(tài) | |
| 物種來源 | |
| 包裝規(guī)格 | 1000ml |
| 是否進(jìn)口 | 是 |
GT-T551培養(yǎng)基適合人T淋巴細(xì)胞（T細(xì)胞）的擴(kuò)增。培養(yǎng)基添加了人白蛋白（醫(yī)藥級別），重組人胰島素，L-谷氨酰胺和鏈霉素，不含其它蛋白和生長因子。不論是否添加血清，GT-T551都能支持細(xì)胞穩(wěn)定生長。
GT-T551培養(yǎng)基支持Anti-CD3/IL-2介導(dǎo)的T細(xì)胞刺激活化。用這種方法激活T細(xì)胞時(shí)，如添加RetroNectin重組人纖維蛋白片段可以增強(qiáng)T細(xì)胞的擴(kuò)增，而且所得T細(xì)胞比單獨(dú)使用CD3單抗產(chǎn)生更多 naïve T cells（初始T細(xì)胞）。
GT-T551 培養(yǎng)基產(chǎn)品特點(diǎn)
日本原裝進(jìn)口無血清培養(yǎng)基，已經(jīng)加入人血白蛋白組分。
支持淋巴細(xì)胞的高密度細(xì)胞培養(yǎng)。
在日本多家醫(yī)院用于臨床 的細(xì)胞培養(yǎng)。
配制用水符合國家藥監(jiān)局《人體細(xì)胞 研究和制劑質(zhì)量控制指導(dǎo)原則》中注射用水標(biāo)準(zhǔn)。
GT-T551 培養(yǎng)液在使用前加入0.6%自體血漿，性能可以和許多高價(jià)培養(yǎng)液效果相同。
GT-T551 H3 是GT-T551的升級版培養(yǎng)基，采用全新的緩沖系統(tǒng)，可用于無血漿培養(yǎng)，CD3+CD56+比例高。如果培養(yǎng)時(shí)添加自體血漿，效果更為顯著。
T細(xì)胞擴(kuò)增方法詳見TAKARA GT-T551說明書。
GT-T551 is a culture medium ideally suited for expansion of human T lymphocytes (T cells). It is supplemented with human albumin (pharmaceutical grade), recombinant human insulin, L-glutamine, and streptomycin; no additional proteins or growth factors are added. The medium can support stable growth in the presence or absence of serum.
Anti-CD3/IL-2 T-cell activation can also be performed in this medium (Wang et al. 2013; Ai et al. 2014; Dodo et al. 2014). When using this activation method, the additional presence of RetroNectin enhances the proliferation of T cells (Yu et al. 2008; Ishikawa et al. 2014; Hosoi et al. 2014; Sakamoto et al. 2015; Li et al. 2015) and results in an expanded cell population that shows a higher proportion of naïve T cells than anti-CD3 antibody stimulation alone (Hosoi et al. 2014).
Features
Used for extended culture or expansion of T cells
Supplemented with human serum albumin, human insulin, L-glutamine, and streptomycin
Endotoxin tested (endotoxin <0.3 EU/ml)
Compatible with CultiLife Culture Bags for large-scale expansion of T cells
Anti-CD3/IL-2 T-cell activation in the presence of RetroNectin enhances proliferation and leads to a higher proportion of naïve T cells. For more information, the user manual can be found under the Documents tab.
The method of T-cell expansion by RetroNectin stimulation requires a license from Takara Bio Inc. for uses other than research.
