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Cellartis Y30045 DEF-CS 500 Xeno-Free Culture Medium 無(wú)外源人多能誘導(dǎo)干細(xì)胞培養(yǎng)基，不含抗生素

英文名稱：Cellartis DEF-CS 500 Xeno-Free Culture Medium without Antibiotics
中文名稱：Cellartis DEF-CS 500 無(wú)外源 iPS 培養(yǎng)基，不含抗生素
產(chǎn)品編號(hào)：HY-001466
訂購(gòu)貨號(hào)：Y30045
生產(chǎn)廠家：TAKARA
規(guī)格：1 Kit

組成：

• 500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium without Antibiotics (Cat. No. Y30046；不單獨(dú)銷售)
• Cellartis DEF-CS 500 Xeno-Free Additives (Cat. No. Y30042)
  • 500 μl DEF-CS Xeno-Free Additive 1 (1000X)
  • 200 μl DEF-CS Xeno-Free Additive 2 (1000X)

特點(diǎn)：

• 完全不含人源及動(dòng)物源成分（xeno-free）
• 不含抗生素
• 無(wú)需飼養(yǎng)層細(xì)胞
• 采用溫和非酶促單細(xì)胞傳代方法
• 支持高增殖率與穩(wěn)定核型
• 保持iPS細(xì)胞多能性、長(zhǎng)期自我更新及分化潛能
• 高重復(fù)性與生長(zhǎng)穩(wěn)定性

用途：適用于臨床前級(jí)別人誘導(dǎo)多能干細(xì)胞（iPS）的擴(kuò)增。

保存條件：基礎(chǔ)培養(yǎng)基 2–8°C；添加劑 –20°C。

配套試劑要求（需另行購(gòu)買）：

• 緩沖液：D-PBS（含Ca2?/Mg2? 與 不含Ca2?/Mg2?）
• 基底：iMatrix-511（Takara Clontech, Cat. No. T303）或 Corning Synthemax II-SC（Cat. No. 3535）
• 消化劑：Versene Solution（Life Technologies, Cat. No. 15040）

Cellartis DEF-CS 500 Xeno-Free Culture Medium without Antibiotics is intended for the expansion of human induced pluripotent stem (iPS) cells in conditions that are completely free from human- and animal-derived components. Cells are cultured in the absence of feeder cells; instead, a xeno-free substrate such as iMatrix-511 is used. The culture protocol uses gentle, non-enzymatic single-cell passaging to ensure controlled and reproducible conditions. Cells cultured in this medium have a high-proliferation rate and stable karyotype, allowing for efficient scale-up. The combination of these features makes this medium ideal for generating preclinical-grade iPS cells for regenerative medicine studies.